CD36 peptides inhibit the binding of rC1-2 [1-179] to CD36. Peptides were prepared in 0.45 mL of binding media pH 6.7 containing 1% BSA and 5% DMSO and microfuged for 5 minutes before addition to the coated beads to remove insoluble peptide material. Peptides were incubated, 1 hour at room temperature with beads coated with rC1-2 [1-179] before addition of sCD36. After 3 hours incubation, the beads were washed and solubilized for SDS-PAGE.17 The binding of CD36 was assayed by Western blot using biotinylated MoAb 179 recognizing a tag incorporated to the sequence of sCD36.17 All samples (except No DMSO) had a 5% final concentration of DMSO. Percent inhibition of binding (from the DMSO control) is indicated at the bottom of the figure. Negative numbers means higher binding than the DMSO control. (A) Various CD36-derived peptides tested at 50 μmol/L. (B) Concentration-dependent inhibition of binding of CD36 to rC1-2 [1-179]. Peptides concentration is in μmol/L. Peptide CD36 139-155 was largely insoluble at 100 μmol/L and peptide CD36 146-164 was only partially soluble at 50 μmol/L. In both cases, most of the peptide was removed by the centrifugation step resulting in low to no inhibition (data not shown).