Fig. 4.
Blockade of PE adherence to immobilized CD36 and ICAM-1. PE from clone A4ultra were incubated for 1 hour in binding media containing the appropriate peptide and final concentration of 1% DMSO, then added to the spotted receptor for 1 hour and processed as described in Materials and Methods. Results are given as percent binding from the control (no peptide). (A) Blockade of adhesion to CD36 tested at 100 μmol/L. MoAb 8A6 was tested at 10 μg/mL. (B) Blockade of adherence to ICAM-1. Peptide CD36 156-184 was tested at 100 μmol/L, peptide ICAM-1 15-20 was tested at 100 and 500 μmol/L. (C) Concentration-dependent blockade of adherence to CD36. Peptide inhibition was tested at 1 to 100 μmol/L range as described in (A).