Fig. 3.
Effects of the NO donor, SIN-1, on hypoxia-induced sickle RBC adherence (A) and VCAM-1 expression (B) on HLMECs. Endothelial cell monolayers were subjected to hypoxia in the presence or absence of the indicated concentrations of SIN-1 for 24 hours and then assessed for sickle RBC adherence and VCAM-1 expression. Values presented are the means ± 1 SD from 8 (RBC adherence) or 3 (VCAM-1 expression) experiments. Differences observed in RBC adhesion (A) and VCAM-1 expression (B) between normoxic, hypoxic, and hypoxic plus SIN-1–treated groups were significantly different as assessed by one-way analysis of variance (P < .01).