Ex vivo intracellular Bcl-2 and Bax protein expression by T cells from UPN 342 at various times after BMT. (A) A follow-up study of Bcl-2 levels in CD4+ and CD8+ T cells from UPN 342 is shown. Percentages of cells with a Bcl-2 content lower than that of donor cells are indicated. (B) Immunoblot analysis of Bcl-2 and Bax protein expression in 5 × 105 T FACS-purified CD4+ and CD8+ T cells from UPN 342. Actin levels were assessed for the same samples. Lane 1, donor T cells; lane 2, ex vivo T cells on day 90 after BMT; lane 3, ex vivo T cells on day 840 after BMT. (C) Relative levels of Bcl-2 and Bax proteins determined by scanning the exposed films with a densitometer and expressed relative to the level of actin in the same samples. Ratios were calculated for each experimental point as follows: absorbance of Bcl-2 or Bax staining/absorbance of actin staining. The ratio for sample 1 was set as 1 arbitrary unit. All the other ratios were referred to this internal ratio.
