Immunohistology of HD tissues. Immunohistology of frozen sections of nodular sclerosis subtype of HD stained for eotaxin using a monoclonal anti-eotaxin antibody (red reaction product-APAAP technique). (A and B) Eotaxin-expressing cells are located within the collagen tissue bands. Hodgkin/Reed-Sternberg cells and other reactive cells are eotaxin negative. (C) Double labeling for CD3 (brown reaction product-streptavidin/biotin method) and eotaxin in a case of nodular sclerosing classical HD shows that most of the spindle-shaped eotaxin-expressing cells do not coexpress CD3.