Induction of eotaxin mRNA expression in normal human dermal fibroblasts. Northern blot analysis of total RNA (10 μg) isolated from fibroblasts. Blots were hybridized with eotaxin- and subsequently with GAPDH-specific probes. (A) Untreated fibroblasts (lane 1), fibroblasts after stimulation with 30 ng/mL TNF- (lane 2), and fibroblasts after cocultivation with the Hodgkin cell lines L1236 (lane 3), L428 (lane 4), KM-H2 (lane 5), and the pre-B–cell line Blin-1 (lane 6). (B) Fibroblasts after stimulation with 10 ng/mL TNF- (lanes 1 and 2) and incubation with anti–TNF- antibodies (lane 2). Fibroblasts after cultivation in supernatant of the Hodgkin cell line L1236 (lanes 3 and 4) and incubation with anti–TNF- antibodies (lane 4).