Th2 lymphocyte chemotaxis in response to recombinant eotaxin and to supernatants of Hodgkin cell lines and fibroblasts. (A) The number of Th2 cells migrated at indicated concentrations of eotaxin (in nanomoles per liter) is given relative to the number of Th2 cells migrated at medium control (no chemokine), which was set arbitrarily at 1 (chemotaxis index). (B and C) (□) Th2 cells migrated in response to supernatants of cell lines indicated; (▩) Th2 cells migrated in response to supernatant of fibroblasts stimulated with TNF- (left panel) or to supernatants of fibroblasts cocultured with cell lines indicated (right panel); (▪) migration of Th2 cells after blocking the chemoattractant eotaxin with anti-eotaxin MoAb. Values of columns are given relative to values of Th2 cells migrated at medium control, which were set arbitrarily at 1 (chemotaxis index). Th2 cells migrated for 2.5 hours (A and B) and for 4 hours (C), respectively. Values of induction and inhibition of specific chemotactic responses are statistically significant for all supernatants (P < .005 using the Student’s t-test). Results are the mean values of 3 independent experiments and errors are shown as the standard deviation.