(A) Phenotype of naive AML blasts and leukemia-derived DC (patient SC). Freshly thawed PBMC from an AML-M2 patient and the same cells after 7 days of culture in the presence of GM-CSF, TNF, and IL-4 were stained with fluorescein-or phycoerythrin-conjugated antibodies and analyzed by flow cytometry. To exclude debris, the viable cells were gated based on propidium iodide staining. Histograms represent the log of fluorescence (horizontal axis) versus the relative cell number (vertical axis). Thin lines represent the isotype-matched indifferent murine MoAb control. The number in each box represents the percentage of positive cells. (B) CD7/CD80 coexpression on naive AML blasts and leukemia-derived DC (patient SC). Cells at day 0 and day 7 of culture were double-stained with fluorescein-conjugated anti-CD7 MoAb (horizontal axis) and anti-CD80 phycoerythrin-conjugated MoAb (vertical axis). Fluorochrome-conjugated isotype-matched murine MoAbs (IgG1) were used as a negative control. The number in each quadrant represents the percentage of positive cells.