Fig. 2.
Fig. 2. (A) Gel-shift analysis with nuclear extracts from HepG2 cells incubated under normoxic (20% O2) or hypoxic (3% O2) conditions for 4 hours in the absence or presence of IL-1β 300 pg/mL. (B) Gel-shift analysis with nuclear extracts from normoxic HepG2 cells and hypoxic HepG2 cells treated with TNF- 10 ng/mL or IL-1β 300 pg/mL for 4 hours. Specificity was demonstrated in a supershift experiment with anti–HIF-1 antibodies (AB). See Fig 1for abbreviations.

(A) Gel-shift analysis with nuclear extracts from HepG2 cells incubated under normoxic (20% O2) or hypoxic (3% O2) conditions for 4 hours in the absence or presence of IL-1β 300 pg/mL. (B) Gel-shift analysis with nuclear extracts from normoxic HepG2 cells and hypoxic HepG2 cells treated with TNF- 10 ng/mL or IL-1β 300 pg/mL for 4 hours. Specificity was demonstrated in a supershift experiment with anti–HIF-1 antibodies (AB). See Fig 1for abbreviations.

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