Fig. 3.
Fig. 3. Detection of HIF-1 protein by Western blot analysis. Twenty micrograms of nuclear protein per lane was sufficient for detection by a monoclonal anti–HIF-1 antibody. IL-1β caused an accumulation of HIF-1 protein in nuclei from HepG2 cells under normoxic and hypoxic conditions. TNF- was ineffective.

Detection of HIF-1 protein by Western blot analysis. Twenty micrograms of nuclear protein per lane was sufficient for detection by a monoclonal anti–HIF-1 antibody. IL-1β caused an accumulation of HIF-1 protein in nuclei from HepG2 cells under normoxic and hypoxic conditions. TNF- was ineffective.

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