Fig. 1.
Fig. 1. Immunoreactivity of the MoAb Kat 6 to multinucleated cells formed in bone marrow culture. Rat bone marrow cells were cultured in the presence of htROSCM and 10−8 mol/L 1,25(OH)2D3 for 5 days. The detached cells were centrifuged onto a glass slide by using a cytospin, fixed with 2% PFA, and stained by MoAb Kat 6 using FITC-conjugated antimouse IgM as the second antibody. Positive signals in the nucleus of the multinucleated cells in the culture are indicated by arrows. (Original magnification × 443.)

Immunoreactivity of the MoAb Kat 6 to multinucleated cells formed in bone marrow culture. Rat bone marrow cells were cultured in the presence of htROSCM and 10−8 mol/L 1,25(OH)2D3 for 5 days. The detached cells were centrifuged onto a glass slide by using a cytospin, fixed with 2% PFA, and stained by MoAb Kat 6 using FITC-conjugated antimouse IgM as the second antibody. Positive signals in the nucleus of the multinucleated cells in the culture are indicated by arrows. (Original magnification × 443.)

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