![Fig. 9. Effects of expressional blockage of OCZF cDNA with antisense ODN on the formation of MNCs, POCs, and macrophage polykaryons. (A) Inhibition of MNC formation with antisense ODN but not sense or scrambled ODN in bone marrow culture. Bone marrow cells were cultured with 10−8 mol/L 1,25(OH)2D3 and htROSCM for the formation of MNCs. After 5 days of culture, TRAP-positive MNCs were counted. (B) Effect of ODN-2 on the formation of POCs. Nonadherent bone marrow cells were cultured in the presence of 10−8 mol/L 1,25(OH)2D3 and htROSCM for the formation of POCs. After 5 days of culture, TRAP-positive mononuclear cells were counted. (C) Effect of ODN-2 on the formation of macrophage polykaryons. Bone marrow cells were cultured with 10−7mol/L PMA and 10−8 mol/L 1,25(OH)2D3. After 4 days of culture, TRAP-negative MNCs were counted. One micromole per liter ofOCZF antisense (A) or sense (S) ODN-1 ([A] experiments no. 1 and 2) or antisense (A), sense (S), or scrambled (Scr) ODN-2 ([A] experiment no. 3, [B], and [C]) was added to the culture. Data are the mean ± SEM of quadruplicate cultures. **P < .01 compared with control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/6/10.1182_blood.v94.6.1987/5/blod41826009x.jpeg?Expires=1735599292&Signature=ganOZKNttvK6Fwqasyv7TGWWt6eLEuXurjt-eMnugHf5m0pta4DJhRXdqolDsLfu4lqENCW2jOoAiTLdqnppx3bsNDpxAfvxLkeETtac3CmkwXRIbz~Wwwyed9e1OWRtXEGjImSwefGUJfiNQF1Lk1W-Ry78VxRm3ZhkH8flezhSCnjiBReHX2X9T3qtZg3ievfuMDSHxvCHOh8xIYHnBb6VFzTQQTktg-wg9umr-8mRCKELI-RFB2IsgP9~F8Y0E5oPG8nJpuE6tutVSGPAFvbICBxmmpNxB5dMwnUA1b~68VfFkmn2lXIywG0wmWrrPCLHfNE4FOFiIk-IMoIQsg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of expressional blockage of OCZF cDNA with antisense ODN on the formation of MNCs, POCs, and macrophage polykaryons. (A) Inhibition of MNC formation with antisense ODN but not sense or scrambled ODN in bone marrow culture. Bone marrow cells were cultured with 10−8 mol/L 1,25(OH)2D3 and htROSCM for the formation of MNCs. After 5 days of culture, TRAP-positive MNCs were counted. (B) Effect of ODN-2 on the formation of POCs. Nonadherent bone marrow cells were cultured in the presence of 10−8 mol/L 1,25(OH)2D3 and htROSCM for the formation of POCs. After 5 days of culture, TRAP-positive mononuclear cells were counted. (C) Effect of ODN-2 on the formation of macrophage polykaryons. Bone marrow cells were cultured with 10−7mol/L PMA and 10−8 mol/L 1,25(OH)2D3. After 4 days of culture, TRAP-negative MNCs were counted. One micromole per liter ofOCZF antisense (A) or sense (S) ODN-1 ([A] experiments no. 1 and 2) or antisense (A), sense (S), or scrambled (Scr) ODN-2 ([A] experiment no. 3, [B], and [C]) was added to the culture. Data are the mean ± SEM of quadruplicate cultures. **P < .01 compared with control.
