Northern analysis of OCZF mRNA from antisense, sense, and scrambled ODN-2–treated bone marrow cells. Bone marrow cells were cultured with 10⁻⁸ mol/L 1,25(OH)₂D₃ and htROSCM for the formation of MNCs in the presence of 1 μmol/L OCZF ODN-2 for 4 days. RNA was isolated from the 4-day culture and approximately 1 μg of each sample was analyzed. Northern analysis was performed as described in Materials and Method. The same filter was rehybridized with a human β-actin probe as a control. Arrows indicate the 5.3- and 3.3-kb bands of OCZF and β-actin mRNA. Lanes correspond to RNA from scramble (lane 1), sense (lane 2), and antisense (lane 3) ODN-treated cells.