ImmunoEM appearance of defensin/Lp(a) complex. Electron micrographs of defensin/Lp(a) complexes. Lp(a) was incubated alone or in the presence of 1:10 molar ratio of defensin for 30 minutes at 22°C followed by incubation with anti-defensin sera for 30 minutes at 22°C. All specimens were rotary shadowed with tungsten. A gallery of selected examples of individual Lp(a) molecules attached to anti-defensin antibody in the presence and absence (lower panel, far right) of defensin is shown. No binding was seen when nonimmune sera was substituted for anti-defensin sera. The arrows denote the location of the anti-defensin antibodies.