Subcellular distribution of defensin, Lp(a), and Lp(a)/defensin complexes in HUVECs as assessed by confocal microscopy. HUVECs incubated with either Lp(a) (50 nmol/L; B) or defensin (10 μmol/L; C) or coincubated with defensin and Lp(a) (D) for 30 minutes at 37°C and then analyzed by confocal microscopy using polyclonal Lp(a) antibodies followed by FITC-conjugated antirabbit IgG (detected as green) and/or a monoclonal antihuman defensin followed by rhodamine TRITC-conjugated donkey antimouse IgG (detected as red). Normal rabbit IgGs served as control, as shown in (A). Cell nuclei were stained with DAPI (detected as blue). Colocalization of defensin and Lp(a) was assessed by double staining (D) and results in a yellow color (arrows).