(A) FACS of the different cells obtained from culture explants of 4 patients. The results are presented in percentages of cells that were fluorescent with anti-vWF and anti-PECAM antibodies. Bars denote standard deviation. The MFI, expressed in arbitrary units of fluorescence, is indicated inside the corresponding histograms. AMEC-1 and AMEC-2 (passages 2 and 3) were positive with anti-vWF, and the same percentage of cells was labeled with anti-CD31. In the further experiments, only AMEC-1 and AMEC-2 were considered as AMEC. (B) Flow cytometry profiles of HUVEC, AMEC-1, and AMEC-2 (passages 2 and 3) obtained with anti-CD31 and anti-vWF antibodies. The results showed that the AMEC were as homogeneous as the HUVEC with the 2 antibodies. The control corresponds to HUVEC or AMEC incubated with FITC conjugated antimouse or antirabbit Igs.