Fig. 5.
Endothelial cell proliferation in presence of IL-1β (20 U/mL), TNF- (500 U/mL), IFN-γ (250 U/mL), or TGF-β (2 ng/mL). The effect of cytokines was tested by using [methyl-3H]thymidine incorporation into HUVEC, HAEC, HMEC-1, and AMEC and was expressed as a percentage of thymidine incorporation inhibition. For HUVEC, HAEC, and HMEC-1, the experiments were done in triplicate with cells obtained from 2 to 4 different cell cultures. For AMEC, the experiments were performed in triplicate with cells obtained after 2 and 3 passages. The results are expressed as means of AMEC-1 and AMEC-2.