Expression of recombinant WT gp91^phoxand ▵488-497 gp91^phox flavocytochromeb₅₅₈ in transgenic PLB-985 cells. X-CGD PLB cells were transfected with either WT or ▵488-497 gp91^phox cDNAs, and the expression of recombinant gp91^phox/p22^phox heterodimer was examined by immunoblotting (B). Five micrograms of cellular membranes was loaded. (A) Confocal microscopy observation of membrane surface expression of recombinant gp91^phox. The indicated cells were stained with the gp91^phoxmonoclonal antibody, 7D5, as described previously,18 and mouse IgG1 was used as an isotype control. Imaging amplifications: ×360 for 7D5 staining and ×148 for IgG1 staining.