American Society of Hematology

Fig. 4.

$Fig. 4. Effect of peptide 488-497 on NADPH oxidase activity in the cell-free oxidase assay. Plasma membrane (8 μg) and cytosolic fractions (20 μg) separated from normal neutrophils were used in the cell-free assay. Peptide 488-497 of gp91phox (•) as well as a control peptide corresponding to residues 86-102 of gp91phox containing a putative p47phox binding site 86-93 (▪) were added to the assay before the addition of SDS, and O2−generation was measured. The activity of superoxide production in the absence of peptides was 216 ± 22 nmol/min/mg of membrane proteins. The data represent the mean ± SD of 3 separate experiments.$

Effect of peptide 488-497 on NADPH oxidase activity in the cell-free oxidase assay. Plasma membrane (8 μg) and cytosolic fractions (20 μg) separated from normal neutrophils were used in the cell-free assay. Peptide 488-497 of gp91^phox (•) as well as a control peptide corresponding to residues 86-102 of gp91^phox containing a putative p47^phox binding site 86-93 (▪) were added to the assay before the addition of SDS, and O₂⁻generation was measured. The activity of superoxide production in the absence of peptides was 216 ± 22 nmol/min/mg of membrane proteins. The data represent the mean ± SD of 3 separate experiments.

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