Detection of vector sequences in peripheral blood and marrow from dog E649 transplanted with CD34-enriched marrow cells transduced by 72 hours on CH-296 (LNY) or BSA (LNX), or by cocultivation (LN). (A) PCR analysis of peripheral blood cells and marrow cells. PB, peripheral blood; BM, bone marrow. (B) Percentage of vector-positive DNA as measured by phosphor image analysis of signal intensities for LN, LNX, and LNY corrected for the amount of DNA as determined by PCR for the β-actin gene. (C) Southern blot analysis.