Fig. 5.
Fig. 5. Inhibition of βc association with 14-3-3ζ by phosphorylated but not by unphosphorylated βc and raf-1 peptides. Lysates of HEK293T cells transfected with βc were immunoprecipitated with GST–14-3-3-sepharose in the absence or in the presence of chemically synthesized peptides (100 μmol/L) containing a βc sequence (CLGPPHSRSLPDILG) or a Raf-1 sequence (CLSQRQRSTSTPNVHM). In the phosphorylated peptides, the relevant phosphorylated serine is underlined. The experiment was performed on 7.5% SDS-PAGE under reducing conditions. The presence of βc in the precipitation experiment was determined by Western blotting with anti-βc antibody (MoAb 1C1).

Inhibition of βc association with 14-3-3ζ by phosphorylated but not by unphosphorylated βc and raf-1 peptides. Lysates of HEK293T cells transfected with βc were immunoprecipitated with GST–14-3-3-sepharose in the absence or in the presence of chemically synthesized peptides (100 μmol/L) containing a βc sequence (CLGPPHSRSLPDILG) or a Raf-1 sequence (CLSQRQRSTSTPNVHM). In the phosphorylated peptides, the relevant phosphorylated serine is underlined. The experiment was performed on 7.5% SDS-PAGE under reducing conditions. The presence of βc in the precipitation experiment was determined by Western blotting with anti-βc antibody (MoAb 1C1).

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