Fig. 4.
Time dependence of LIP recovery and total ferritin levels in K562 cells after iron chelation. K562 cells were treated for 10 minutes with 100 μmol/L SIH and then incubated in serum-free growth medium alone (▪) or containing leupeptin 20 μg/mL (added at 0 time and at 8 hours; ▩). At the indicated times, cells were washed and analyzed for LIP (by the calcein method) and total ferritin levels (by ELISA) as described in Fig 3. Ferritin levels (upper panel) are given relative to untreated cells (extreme left bar) and LIP levels (lower panel) expressed in micromoles per liter.