Fig. 1.
Fig. 1. Mobility shift of the γ subunit of FcɛRI after cross-linking of FcRI. The γ subunit was immunoprecipitated from lysates of FcRI (lanes 2 and 3) or FcγRI (lanes 4 and 5) cross-linked U937IF cells as described in Materials and Methods. Lane 1 represents immunoprecipitation using rabbit IgG of U937IF cells stimulated with anti-FcγRI and rabbit antimouse F(ab′)2 antibody (RM) for 1 minute. Resting U937IF cells were immunoprecipitated with anti-γ antibodies (lanes 2 and 4). Anti-γ immunoprecipitates of U937IF cells stimulated with anti-FcR (A77) after RM (both Fab′2 fragments) for 1 minute (lane 3) or with anti-FcγRI after RM for 1 minute (lane 5). Lane 6 is a whole cell lysate prepared from U937IF cells stimulated with anti-FcγRI antibody after RM for 1 minute. γ0 and γ1 represent the baseline and mobility shifted isoforms of FcɛRIγ subunit, respectively.

Mobility shift of the γ subunit of FcɛRI after cross-linking of FcRI. The γ subunit was immunoprecipitated from lysates of FcRI (lanes 2 and 3) or FcγRI (lanes 4 and 5) cross-linked U937IF cells as described in Materials and Methods. Lane 1 represents immunoprecipitation using rabbit IgG of U937IF cells stimulated with anti-FcγRI and rabbit antimouse F(ab′)2 antibody (RM) for 1 minute. Resting U937IF cells were immunoprecipitated with anti-γ antibodies (lanes 2 and 4). Anti-γ immunoprecipitates of U937IF cells stimulated with anti-FcR (A77) after RM (both Fab′2 fragments) for 1 minute (lane 3) or with anti-FcγRI after RM for 1 minute (lane 5). Lane 6 is a whole cell lysate prepared from U937IF cells stimulated with anti-FcγRI antibody after RM for 1 minute. γ0 and γ1 represent the baseline and mobility shifted isoforms of FcɛRIγ subunit, respectively.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal