Detection of c-kit receptor activation and MMP-9 secretion of IC-2 transfectants and BMCMCs. SCF-dependent activation of the c-kit receptor was observed in BMCMCs and IC-2^WT cells, whereas SCF-independent phosphorylation was detected in a constitutively active c-kit transfectant (IC-2^V814; A). ProMMP-9 activity is marked in control IC-2^VECTOR cells and IC-2^WT cells as well as the original IC-2 cells, but not in IC-2^V814 cells even when stimulated with TPA (B).