MoAb BION-1 inhibits IL-5–, GM-CSF–, and IL-3–stimulated but not TNF-stimulated CD69 upregulation on human eosinophils. (A) CD69 upregulation in the presence of different concentrations of IL-5 (▪), GM-CSF (•), IL-3 (▴), and TNF (⧫). (B) CD69 upregulation stimulated by 0.3 nmol/L of IL-5, GM-CSF, IL-3, or TNF in the presence of different concentrations of MoAb BION-1 (open symbols) or control anti-β_c MoAb, 8E4 (solid symbols). Results are expressed as the percentage increase or change in CD69 expression relative to unstimulated cells. Each point is the mean value of 3 replicates and error bars represent 1 standard deviation.