Comparison of fibrinogen and D dimer induction of AP-1–DNA binding activity by EMSA. Fibroblasts were incubated with either fibrinogen or D dimer (1 μmol/L) for 45 minutes in serum-free media, followed by harvesting of nuclear extracts, which were analyzed by EMSA using a ³²P-labeled PAIcon (A, left) or PAIwt (A, right) oligonucleotides. Complex B band density was quantified on a phosphorimager and plotted as mean +SE (n = 3/condition) in (B). Serum-free media or serum (10%) -containing media-exposed cells served as negative and positive controls, respectively. DDex, FGNex, 10%Sex, and SFMex denote nuclear extracts from D dimer, fibrinogen, 10% serum-containing media, and serum-free media-exposed cells, respectively. 100X PAIcon denotes inclusion of a 100-fold molar excess of unlabeled PAIcon oligonucleotides in the binding reaction. (A) Left, EMSA using the ³²P-labeled PAIcon oligonucleotide; right, EMSA using the ³²P-labeled PAIwt oligonucleotide. Complex B is indicated. (B) Quantitative presentation of complex B band density (AP-1). (□) Data from ³²P-labeled PAIcon oligonucleotide; (▪) ³²P-labeled PAIwt oligonucleotide.