(A) Side scatter and forward scatter characteristics of unstimulated eosinophils. (B) Time course for the SA-mediated eosinophil apoptosis. Eosinophils were incubated with medium control or 50 μmol/L SA for 2 to 19 hours as indicated. Original flow cytometric histograms demonstrating hypodiploid DNA peaks representing apoptotic eosinophil nuclei are demonstrated. SA-mediated apoptosis (unfilled graph) occurred after about 8 hours similar to Fas-mediated eosinophil apoptosis (not shown) when compared with spontaneous apoptosis (medium control, shown by the filled graph).