Fig. 2.
![Fig. 2. Stage-specific suppression of CFU-Meg by TGF-β1. CFU-Meg colony assay was performed with CD34+ cells incubated with TGF-β1 (1.0 ng/mL) in a delayed addition fashion at days 0, 2, 4, 6, 8, 10, 12, and 14 ([▩] presence of TPO in culture medium; [▧] presence of TGF-β1 in the culture medium). CFU-Meg colony assays, without TGF-β1 and with TGF-β1 preincubation for 24 hours, were also performed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/6/10.1182_blood.v94.6.1961/5/blod41817002x.jpeg?Expires=1735522741&Signature=GuJFNGo8jUgNWLH~akakpToChXjFS0JbDkkrMhIrGrJAeRVKED2wiH8dbDGV8p9s99tKbXNirkaN2GLb7HcLl0Sr4SoHmPdHdh01V9QW5Sl6p66Ty681JPkYgUxSfMq1zhDjWr4uZ8bWBCNzQeQG5hz0KVxAXk~DKkbcW-enPDbZ5IhDrm3fnRSNwN7XyIqkmOX~X~u1YbeMJti7UMZUUbW5sv5jqyyGZ-q~IYla0rHhRiw8a3y1cy7gv-3eBg5XT4DJI09MapE5bOnC7MecJ9YbaGMlxIcMBRB3kOUMpjk5Yev5HF6iEkxHaXzFmP-865kTfDYYQkuTBTKk-NAViA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stage-specific suppression of CFU-Meg by TGF-β1. CFU-Meg colony assay was performed with CD34+ cells incubated with TGF-β1 (1.0 ng/mL) in a delayed addition fashion at days 0, 2, 4, 6, 8, 10, 12, and 14 ([▩] presence of TPO in culture medium; [▧] presence of TGF-β1 in the culture medium). CFU-Meg colony assays, without TGF-β1 and with TGF-β1 preincubation for 24 hours, were also performed.
