Southern blot analysis of the 5′ region of the EphA3 gene in normal human samples of lymphocytes and monocytes isolated from cord blood, adult pancreas, and placental tissues. Genomic DNA (10 μg) from each sample was digested withSacI (S) or double digested with SacI and AvaI (SA), SacI and PvuII (SP), and SacI andSacII (SS). AvaI and SacII are methyl-sensitive restriction enzymes. The Southern blot was hybridized with the 1.4-kbSacI genomic fragment containing the EphA3 promoter.