Flow cytometric analysis of CD4 and CD8 expression on thymocytes and splenocytes of WT and Tg-KIR2DL3 × HLA-Cw3 mice. Freshly isolated thymocytes and splenocytes from WT and Tg-KIR2DL3 × HLA-Cw3 mice were stained with anti-CD4 and anti-CD8 MoAbs and analyzed by flow cytometry.