Fig. 1.
(A) IL-7R and IL-3R are coexpressed by a minor fraction of wild-type and Jak3-deficient bone marrow cells. Bone marrow cells were obtained from wild-type (WT) or Jak3−/−(−/−) and were stained, fixed, and analyzed by flow cytometry. One hundred and fifty thousand cells were analyzed in a lymphoid gate defined by scatter criteria. (B) Staining with isotype control antibodies for anti–IL-7R and anti–IL-3R antibodies is shown for wild-type and Jak3−/− lymphoid-gated cells from bone marrow. The numbers in each quadrant indicate the percentage of positive cells in total bone marrow.