Fig. 1.
Thymus-specific expression of GPR-9-6 and comparison of the primary structure of GPR-9-6 with other chemokine receptors. (A) Human immune system multiple-tissue Northern blot II and human multiple-tissue Northern blot were sequentially hybridized to GPR-9-6 cDNA and human β-actin probes at 68°C for 1 hour, washed with 0.1X standard saline citrate (SSC) at 50°C for 40 minutes, and exposed to x-ray films for 12 hours. (B) An alignment of GPR-9-6 with CCR7, CCR6, CCR5, and CCR1 with the use of GeneWork 2.5.1. Putative transmembranes are indicated by overlines.