American Society of Hematology

Fig. 4.

$Fig. 4. Fractionation of transgenic bone marrow cells by hCD4 level. Flow cytometric analysis of cells from a HS21/45vav-hCD4 mouse incubated in the absence (A) or presence (B) of a labeled anti-hCD4 MoAb. Cells within windows a, b, and c were sorted for clonogenic assays. In the representative experiment shown (1 of 3), 8% of viable nucleated cells fell in fraction a (low to negative for surface hCD4), 42% in fraction b (intermediate hCD4), and 50% in fraction c (high hCD4). Most erythrocytes fell in fraction a and the dead cells (9% of the total cells) in fraction d.$

Fractionation of transgenic bone marrow cells by hCD4 level. Flow cytometric analysis of cells from a HS21/45vav-hCD4 mouse incubated in the absence (A) or presence (B) of a labeled anti-hCD4 MoAb. Cells within windows a, b, and c were sorted for clonogenic assays. In the representative experiment shown (1 of 3), 8% of viable nucleated cells fell in fraction a (low to negative for surface hCD4), 42% in fraction b (intermediate hCD4), and 50% in fraction c (high hCD4). Most erythrocytes fell in fraction a and the dead cells (9% of the total cells) in fraction d.

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