Effects of oligonucleotides on differentiation of CD34⁺ cells induced with FL plus IL-3 assayed by morphology. Cells at an initial concentration of 5,000/mL were incubated in medium supplemented with FL at 50 ng/mL and IL-3 at 25 ng/mL to promote monocytic differentiation. On days 0, 5, and 10, antisense RB oligonucleotides (AS2), sense-orientated oligonucleotides (SO2), or scrambled oligonucleotides (SCR) were added, each time at a concentration of 5.0 μmol/L. After 14 days, cytospin slides were prepared and stained with May-Grünwald-Giemsa. Cells incubated with FL, IL-3, and oligo buffer (A); with FL, IL-3, and SO2 (B); with FL, IL-3, and SCR (C); or with FL, IL-3, and AS2 (D).