Fig. 5.
Correlation between replication behavior of the CD34+/CD38− cells in the first 4 days with the growth pattern of the corresponding single cell after 14 days of culture. Wells containing cells showing asymmetric divisions (Asym) were compared with those showing only symmetric divisions (Sym) in their abilities to form BC, reflected in dispersed and mixed growth patterns, and their abilities to form CC after a total of 14 days of culture. The figure summarizes the results of 9 experiments. The percentages of the cells that gave rise to colonies versus those cells that have initially divided are shown. Although the colony data varied widely among samples, the paired t-test showed a significant decrease of BC among cells showing symmetric divisions versus those showing asymmetric divisions. The bars represent the corresponding medians.