Fig. 1.
Enhanced proliferation of BCBL-1 and BC-1 cell lines in response to autologous conditioned medium. The cell lines BCBL-1 and BC-1 in exponential growth phase (5 × 103 and 10 × 103/0.2 mL flat-bottom microwell, respectively) were cultured for 3 days in RPMI 1640 medium supplemented with either fresh medium alone or medium that had previously been conditioned for 24 hours by either the autologous (BCBL-1 or BC-1; ▪) or control (murine AdmEm5; □) cells seeded at 2 × 106 cells/mL in tissue culture flasks. 3H thymidine was added during the final 18 hours of culture. The results represent the mean radioactivity (±SD) of triplicate cultures. Shown is a representative experiment of 9 performed.
