Specificity of GH targeting to WPB. (A) GH (red) versus LAMP-1 (green). GHrAd-infected HUVEC were fixed, permeabilized, and stained with rabbit anti-GH and mouse anti-LAMP-1 (H4A3) antibodies, followed by rhodamine-conjugated antirabbit and FITC-conjugated antimouse antibodies. Several images were obtained by scanning confocal microscopy at 0.36-μm intervals in the Z-axis and then combined into a single image. (B) IG (red) versus vWF (green). IGrAd-infected HUVEC were fixed, permeabilized, and stained with rhodamine-conjugated antihuman IgG and FITC-conjugated anti-vWF antibodies. Shown is a representative confocal scan from the approximate midportion of the Z-axis. (C) GH (red) versus IG (green). HUVEC transduced with both GHrAd and IGrAd were fixed, permeabilized, and stained with rabbit anti-GH antibody and biotinylated goat antihuman IgG antibody, followed sequentially with rhodamine-conjugated goat antirabbit antibody and FITC-conjugated avidin. Shown is a representative confocal scan from the approximate midsection of the Z-axis. In each of these images, there are few yellow granules, indicating minimal colocalization of proteins in each case.