Fig. 1.
![Fig. 1. Identification and purification of the Btk-SH2 domain-binding protein. (A) Lysates of RAMOS cells stimulated with anti-μ-antibody (+) or without stimulation (−) were incubated with the fusion protein of GST and the wild-type Btk-SH2 [SH2(Wild)] or mutated Btk-SH2 [SH2(R307K)] domain. Binding phosphoproteins were detected by immunoblotting with the antiphosphotyrosine (anti-pTyr) antibody 4G10. (B) Ponceau S staining of the purified proteins on PVDF membrane. (C) Peptide mass map obtained by MALDI mass analysis. The result obtained from the peptide mixture generated byAchromobacter protease I digestion of the 68-kD protein is representatively shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/7/10.1182_blood.v94.7.2357.419k40_2357_2364/6/blod41940001w.jpeg?Expires=1735504059&Signature=4RcFhQpHLisae5Wg0l8Q0SBSt7Flio~1IKLWweCHH7T0FAI~Js--Dvfa8PXXC7KUT7WPzXOa7BsTPLUA~Uy~bHqP1LJ~-seMMa2q3OUyWh6CsR7YX6MABcRJjI4Hee5CNpETB4F3L1OsAsMx~uFS2ydrjOnZ69FlzWnDMpeqMyKeoCl8ySsUTekLO3T0RRCCo-lUvAwyTifu~OytCFsMLvKB-s6fDFr-WyU9LZEiWoyeTKm3z-ThKYd35gBb2B9Xfv4mmRG9X8u7aAspRH~v7o0n1zJYIfqASjV6NFTvWazjFLRPvj14BDhBLon7dnuHO-wECViqhY6u~U-e7tr4xw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Identification and purification of the Btk-SH2 domain-binding protein. (A) Lysates of RAMOS cells stimulated with anti-μ-antibody (+) or without stimulation (−) were incubated with the fusion protein of GST and the wild-type Btk-SH2 [SH2(Wild)] or mutated Btk-SH2 [SH2(R307K)] domain. Binding phosphoproteins were detected by immunoblotting with the antiphosphotyrosine (anti-pTyr) antibody 4G10. (B) Ponceau S staining of the purified proteins on PVDF membrane. (C) Peptide mass map obtained by MALDI mass analysis. The result obtained from the peptide mixture generated byAchromobacter protease I digestion of the 68-kD protein is representatively shown.
