Tyrosinephosphorylation of PLCγ2 by Btk and Syk on BLNK. Indicated proteins were expressed in 293T cells. Tyrosinephosphorylation of PLCγ2 were detected by immunoprecipitating (IP) with the anti-PLCγ2 antibody followed by immunoblotting with the antiphosphotyrosine antibody 4G10 (top panel). The same filter was reprobed with the anti-PLCγ2 antibody to confirm the equality of the amounts of the PLCγ2 protein (middle). The expression of Btk in the cell lysates is also shown (bottom).