American Society of Hematology

Fig. 3.

$Fig. 3. Flow cytometry detection of mCD24+ cells in the white blood cell buffy coat fraction of peripheral blood at about 6 weeks posttransplant with CD34+ PBSC transduced with MFGS-mCD24 (shaded histograms). The erythrocytes in a sample of whole blood were lysed and the leukocytes labeled with anti-mCD24 as outlined in Materials and Methods. The level of background labeling is delineated by similar analysis of whole blood leukocytes from a nontransplanted control animal (open histograms).$

Flow cytometry detection of mCD24⁺ cells in the white blood cell buffy coat fraction of peripheral blood at about 6 weeks posttransplant with CD34⁺ PBSC transduced with MFGS-mCD24 (shaded histograms). The erythrocytes in a sample of whole blood were lysed and the leukocytes labeled with anti-mCD24 as outlined in Materials and Methods. The level of background labeling is delineated by similar analysis of whole blood leukocytes from a nontransplanted control animal (open histograms).

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