(A) RT-PCR analysis of TIMP-1 and TIMP-2 mRNA levels in CEM cells stimulated with fibronectin. cDNA obtained from resting CEM cells (lane 1) or stimulated with soluble fibronectin at 10 μg/mL for 4 hours (lane 2). β2-microglobulin amplification is used to control template quantity in each condition. (B) Membrane expression of TIMP-1 and TIMP-2 in CEM cells treated with fibronectin-derived peptides. Flow cytometry analysis of CEM cells cultured with a combination of GRGDSPC and EILDVSPT in solution at 100 μg/mL for 16 hours. The peptide GRGES was used as control. Histograms show distribution of fluorescence intensity in 5 × 10³ cells per condition.