(A) Induction of MMP-14 expression in CEM cells by fibronectin. MMP-14 (MT1-MMP) transcripts obtained from baseline CEM cells (lane 1) and CEM cells exposed to fibronectin (10 μg/mL) for 4 hours (lane 2). Concomitant amplification of β2-microglobulin shows equivalent amounts of template in both samples. Lane 3 shows the Nco I restriction fragments of the 828 bp PCR product. (B) Surface expression of MMP-14 induced by fibronectin-derived peptides in CEM cells. CEM cells were incubated with either GRGDSPC, EILDVSPT, a combination of both, or the control peptide GRGES at 100 μg/mL for 16 hours and subjected to flow cytometry. Histograms show fluorescence intensity distribution among 5 × 103 cells immunostained with the polyclonal antibody Ab-2 recognizing MMP-14.
