American Society of Hematology

Fig. 6.

$Fig. 6. Identification of DNase I hypersensitivity site cluster at −147 −154 kb in upstream Kit region. Nuclei were prepared from +/+ BMMC, HCD57 cells, melan-a cells and liver, and digested with DNaseI for increasing periods of time. Subsequently, DNA was restricted with Sac1, fractionated by electrophoresis and analyzed by blot hybridization using different probes. The Sal-Eco, Sal-Sac, and Eco-Eco hybridization probes derived from a Sac1 restriction fragment at −147 −154 kb detected a cluster of hypersensitive sites in mast cells (BMMC) and two sites in melan-a cells in the vicinity of the 5′W57 breakpoint. DNA from liver nuclei contained no hypersensitive sites in this region. Size markers are indicated. A schematic representation of the hypersensitive sites in the distal Kit promoter region around −147 −154 kb is shown below.$

Identification of DNase I hypersensitivity site cluster at −147 −154 kb in upstream Kit region. Nuclei were prepared from +/+ BMMC, HCD57 cells, melan-a cells and liver, and digested with DNaseI for increasing periods of time. Subsequently, DNA was restricted with Sac1, fractionated by electrophoresis and analyzed by blot hybridization using different probes. The Sal-Eco, Sal-Sac, and Eco-Eco hybridization probes derived from a Sac1 restriction fragment at −147 −154 kb detected a cluster of hypersensitive sites in mast cells (BMMC) and two sites in melan-a cells in the vicinity of the 5′W⁵⁷ breakpoint. DNA from liver nuclei contained no hypersensitive sites in this region. Size markers are indicated. A schematic representation of the hypersensitive sites in the distal Kit promoter region around −147 −154 kb is shown below.

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