Fas-L is not expressed in HIV-gag-tetramer positive circulating CD8⁺ T cells, but granzyme A is. Representative results from an A2-expressing seropositive subject are shown. PBMC were costained with Streptavidin PE-conjugated HLA-A2-gag tetramer, CD8-Cy5 and fasL or granzyme A MoAbs. The first 3 panels depict staining of gated CD8 bright lymphocytes; the last panel depicts all cells in the lymphocyte gate to show that most CD8⁻lymphocytes do not stain for granzyme A. The percent of dually positive cells is shown.