Fig. 5.
![Fig. 5. Autoradiogram showing phosphoproteins generated by protein kinase(s) present in immobilized erythrocyte immunoprecipitates. Sephacryl S-300 column fractions no. 40 through 51, combined and concentrated 6-fold, were incubated with agarose-linked antiphosphotyrosine (PT66) in the presence of low ionic strength buffers. Immobilized immunoprecipitates were incubated with protein kinase buffer augmented with 1 μmol/L [γ-32P]ATP (4 × 107 dpm) and 20 mmol/L magnesium acetate. The 12-, 30-, 36-, 42-, 60-, 72-, and 165-kD phosphoproteins are indicated on the right. Molecular mass markers (6.5 to 204 kD) are on the left.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/8/10.1182_blood.v94.8.2778.420k10_2778_2789/7/blod42010005w.jpeg?Expires=1735512333&Signature=yXgeOlpTO1ZWdYzjD-bi1Y5gkzL7q7D2hj7DSc7rbK4wmHVV~2xoQiQ8KTwFojdbUg8tzbNhzVjLcGS5vyV-1DXcuY4a8EmTogV2~MUFY1fgGX84SsOxmeg7WL50Gxlm91tuJzTPWZukcUp3PSmoUo5GjTYzKNzCn3rP3GP2ocAzei5dxWF2vCZdnxWjgfL~JppdrS9KIaN1srY93tjFnF5vS-lwX~8qWINYRqBJ5-YP2lLf4u-RrUcHS0UvKOzIETa0z6iT7gMnhux8yrbhwse0FjloGe3XkdnDT3H1piF3gqnksbB0fMpGqaACUJ~UwLF~rw3lOM-oRDsQqtMo9w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Autoradiogram showing phosphoproteins generated by protein kinase(s) present in immobilized erythrocyte immunoprecipitates. Sephacryl S-300 column fractions no. 40 through 51, combined and concentrated 6-fold, were incubated with agarose-linked antiphosphotyrosine (PT66) in the presence of low ionic strength buffers. Immobilized immunoprecipitates were incubated with protein kinase buffer augmented with 1 μmol/L [γ-32P]ATP (4 × 107 dpm) and 20 mmol/L magnesium acetate. The 12-, 30-, 36-, 42-, 60-, 72-, and 165-kD phosphoproteins are indicated on the right. Molecular mass markers (6.5 to 204 kD) are on the left.
