Fig. 1.
JK cells fail to undergo growth arrest in response to TGF-β. Mv1Lu (•) or JK (○) cells were incubated in the absence or presence of increasing concentrations of TGF-β1 (0 to 20 ng/mL) for 48 hours at 37°C. During the final 4 hours of agonist treatment, cellular DNA was radiolabeled by addition of [3H]thymidine in the culture medium. Upon completion of agonist stimulations, the cells were harvested and prepared for scintillation counting to determine radionucleotide incorporation into cellular DNA as described in Materials and Methods. Data are the mean (± standard error of mean [SEM]) of 3 independent experiments normalized to untreated controls.