Flow cytometric analysis of peripheral blood leukocytes from recipients of allogeneic TCD BM and irradiated allogeneic splenocytes. Peripheral blood was obtained from B10.BR recipients of 0.5 × 10⁶ CD45.2⁺C57.BL6 TCD BM cells and 50 × 10⁶ irradiated CD45.1⁺ C57.BL6 splenocytes. The conditions of the transplant are described in the legend to Fig 6. Upper panels: the total percentages of donor T cells (upper right quadrant), host-type T cells (lower right quadrant), and host non-T cells (lower left quadrant) and donor-type non-T cells (upper left quadrant) are shown for 2 representative animals bled on day +30 and day +112 posttransplant. Lower panels: the fraction of donor T cells derived from the irradiated splenocytes (CD45.1⁺, upper right quadrant) or from the donor BM (CD45.1⁻, lower right quadrant) are shown.