Fig. 4.
![Fig. 4. Effect of cytoplasmic domain deletion on receptor internalization. Cell surface glycoproteins were labeled with [3H]galactose, and receptor internalization was determined as described in Materials and Methods. The values shown are the average of 2 independent determinations. (□) The percentage of surface EPO-R internalized in 40 minutes at 37°C in the absence of EPO (−EPO); (▪) the percentage of surface EPO-R internalized in 40 minutes at 37°C in the presence of EPO (+EPO). Total cell surface glycoproteins internalization was determined from anti–EPO-R immunoprecipitates of lysates from (3H)galactose-labeled parental 32D cells that do not contain an EPO-R (total surface GP).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/8/10.1182_blood.v94.8.2667.420k27_2667_2675/7/blod42027004x.jpeg?Expires=1735581640&Signature=WZFgK04LhUJjub30T2WkO78Ztb2MBw0McDcwFQsYzEM06jUDJJ0mYgcQUww0lcvkgligEyLUYdKWYyTc35~D4EI7OsPuSYe4AJcM12O3C5zbNT9iFm0z7Ci8fUXA50sgprOOB0L-dqSjoruMQtpxC7PR-R~1vGcxe2iNpSLaWdMEy37E8IentIoWqKu9Z3WTq38I3AReqkhulwk6oK0rP96bYyXGUeO9zBSV8IStu~L4CTVmFs-2pNX-ASwtW35fObIqelHX~Yo-w2lhygclzFWe2XPXBsD7LS7s6LZANsEVkpnwgSfNbCHor6JTBg~nKJvllBiC3PBPvltwebBZ0Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of cytoplasmic domain deletion on receptor internalization. Cell surface glycoproteins were labeled with [3H]galactose, and receptor internalization was determined as described in Materials and Methods. The values shown are the average of 2 independent determinations. (□) The percentage of surface EPO-R internalized in 40 minutes at 37°C in the absence of EPO (−EPO); (▪) the percentage of surface EPO-R internalized in 40 minutes at 37°C in the presence of EPO (+EPO). Total cell surface glycoproteins internalization was determined from anti–EPO-R immunoprecipitates of lysates from (3H)galactose-labeled parental 32D cells that do not contain an EPO-R (total surface GP).
