American Society of Hematology

Fig. 1.

$Fig. 1. Purification of Sca-1+Lin− BM cells. Cells in the different cell fractions obtained by MACS purification (see Materials and Methods) were analyzed by FACS for the presence of Sca-1+Lin+, Sca-1+Lin−, and Sca-1−Lin+ cells. Nonspecific staining was determined by FITC- and PE-conjugated isotype control antibodies. Percentage of each subpopulation is shown in the appropriate area of the dot plot.$

Purification of Sca-1⁺Lin⁻ BM cells. Cells in the different cell fractions obtained by MACS purification (see Materials and Methods) were analyzed by FACS for the presence of Sca-1⁺Lin⁺, Sca-1⁺Lin⁻, and Sca-1⁻Lin⁺ cells. Nonspecific staining was determined by FITC- and PE-conjugated isotype control antibodies. Percentage of each subpopulation is shown in the appropriate area of the dot plot.

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