Depletion by MACS of CD8⁺, CD4⁺, CD11b⁺, and CD45/B220⁺cells after removal of Lin⁺ cells from the Sca-1⁺ BM cell fraction. Levels of CD8⁺, CD4⁺, CD11b⁺, and CD45/B220⁺cells in unseparated, Sca-1⁺, and Sca-1⁺Lin⁻ BM cell fractions, obtained by MACS, were analyzed by double staining with antibodies directed against Sca-1 and the above lineage markers. Nonspecific staining was determined by FITC- and PE-conjugated isotype control antibodies.